Reproductive Biology, Sperm storage, and Sexual Maturity of Thamnodynastes strigatus (Serpentes: Dipsadidae).

Life history strategies determine and influence many aspects of species fitness. In this study, we describe the reproductive biology - reproductive cycle, sperm storage, and sexual maturity - of Thamnodynastes strigatus in South Brazil. We analyzed 49 individuals (25 males and 24 females) from herpetological collections. The reproductive cycle of males and females was described considering the morpho-anatomical and histological changes in the testes, ductus deferens, and kidney, as well in the ovary and oviduct. The age at the onset of sexual maturity was determined by skeletochronology of the caudal vertebra. The reproductive cycle is seasonal semi-synchronous and most individuals have a reproductive peak in spring and summer. The seasonal biennial reproductive cycle and viviparity are two phylogenetically conserved characters in Tachymenini snakes. Thamnodynastes Strigatus females store sperm in the utero-vaginal junction furrows during autumn. There were no differences between the ages of sexual maturity of males (4-11y) and females (4-12y). Females reach sexual maturity at larger body sizes, and this may confer an adaptive advantage due to a higher fecundity potential. Herein, we confirmed the previously described seasonal biennial reproductive cycle of T. strigatus through histological analysis.

Reproductive biology, sperm storage, and sexual maturity of Thamnodynastes strigatus (Serpentes: Dipsadidae)

Life history strategies determine and influence many aspects of species fitness. In this study, we describe the reproductive biology - reproductive cycle, sperm storage, and sexual maturity - of Thamnodynastes strigatus in South Brazil. We analyzed 49 individuals (25 males and 24 females) from herpetological collections. The reproductive cycle of males and females was described considering the morpho-anatomical and histological changes in the testes, ductus deferens, and kidney, as well in the ovary and oviduct. The age at the onset of sexual maturity was determined by skeletochronology of the caudal vertebra. The reproductive cycle is seasonal semi-synchronous and most individuals have a reproductive peak in spring and summer. The seasonal biennial reproductive cycle and viviparity are two phylogenetically conserved characters in Tachymenini snakes. Thamnodynastes Strigatus females store sperm in the utero-vaginal junction furrows during autumn. There were no differences between the ages of sexual maturity of males (4-11y) and females (4-12y). Females reach sexual maturity at larger body sizes, and this may confer an adaptive advantage due to a higher fecundity potential. Herein, we confirmed the previously described seasonal biennial reproductive cycle of T. strigatus through histological analysis.

First record of growth rings for 11 native subtropical anuran species of South America.

Skeletochronology is the most accurate method to estimate a population age structure. The methodology is based on the analysis of secondary bone tissue in order to count growth rings. This study aimed to provide initial data, allowing researchers to further work out in the age of individuals and populations, sampling evidence of the presence of growth rings in 11 native species (representing nine families) of a subtropical region of southern Brazil. Four bone samples of each specimen were used to perform the skeletochronological analysis: the penultimate phalanges of the 3rd and 4th fingers, the humerus, and the femur. The presence of growth rings was confirmed in the periosteal layer of the bones of all analyzed species. In comparison with phalanges, growth rings of humeri and femora are more irregular and less distinguishable. This is the first record of growth rings to the native species herein analised. The skeletochronology was proved to be an effective tool in determining the age of anuran amphibians from a subtropical region, since this environment presents well defined climatic seasonality.

Reproductive phenology of the American Bullfrog in subtropical Brazil: photoperiod as a main determinant of seasonal activity.

The North American bullfrog Lithobates catesbeianus continues to invade ecosystems worldwide, potentially causing population declines and even extinctions. Within its native distribution, bullfrogs show prolonged reproductive seasons and high fertility. However, data on breeding biology of bullfrogs ex-situ in invaded localities mainly comes from anecdotal reports. Understanding how invasive species are adjusting their life histories to new colonized environments is important for conservation purposes. Here we describe temporal and spatial abundance, calling activity, spawning and tadpole distribution of bullfrogs in southern Brazil. Eighteen samplings occurred during one year. The abundance of individuals was positively related to longer photoperiods and higher temperatures. Reproductive activity was also positively associated with longer photoperiods. Calling sites, spawning and tadpoles were associated with microhabitats presenting hydrophytes, which may provide shelter and thermal stability to bullfrogs. The reproductive seasonal activity of bullfrogs can be highly variable across its growing geographical range, but in subtropical Brazil it is associated with photoperiod, a highly predictable abiotic determinant. In our study area, bullfrogs presented a breeding season twice as long as that observed in some native localities. We suggest that management strategies directed to bullfrog populations must consider the habitat structures and seasonal regimes determined by each invaded environment.

Molecular and sensory mechanisms to mitigate sunlight-induced DNA damage in treefrog tadpoles.

The increased incidence of solar ultraviolet B (UVB) radiation has been proposed as an environmental stressor, which may help to explain the enigmatic decline of amphibian populations worldwide. Despite growing knowledge regarding the UV-induced biological effects in several amphibian models, little is known about the efficacy of DNA repair pathways. In addition, little attention has been given to the interplay between these molecular mechanisms with other physiological strategies that avoid the damage induced by sunlight. Here, DNA lesions induced by environmental doses of solar UVB and UVA radiation were detected in genomic DNA samples of treefrog tadpoles (Hypsiboas pulchellus) and their DNA repair activity was evaluated. These data were complemented by monitoring the induction of apoptosis in blood cells and tadpole survival. Furthermore, the tadpoles' ability to perceive and escape from UV wavelengths was evaluated as an additional strategy of photoprotection. The results show that tadpoles are very sensitive to UVB light, which could be explained by the slow DNA repair rates for both cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6,4) pyrimidone photoproducts (6,4PPs). However, they were resistant to UVA, probably as a result of the activation of photolyases during UVA irradiation. Surprisingly, a sensory mechanism that triggers their escape from UVB and UVA light avoids the generation of DNA damage and helps to maintain the genomic integrity. This work demonstrates the genotoxic impact of both UVB and UVA radiation on tadpoles and emphasizes the importance of the interplay between molecular and sensory mechanisms to minimize the damage caused by sunlight.

Seasonal, daily activity, and habitat use by three sympatric pit vipers (Serpentes, Viperidae) from southern Brazil.

Viperid snakes are widely distributed in the South America and the greater distribution range of the family is found at the Crotalinae subfamily. Despite the abundance of this snakes along their geographic distribution, some ecological aspects remain unknown, principally at subtropical areas. In the present study, we evaluated the activity (daily and seasonal) and the use of the habitat by Bothrops diporus, B. jararaca and B. jararacussu, in an Atlantic Forest area at southern Brazil. We observed higher incidence of viperid snakes during the months with higher temperatures, while no snakes were found during the months with lower temperatures. The data suggest the minimum temperature as environmental variable with the greatest influence on the seasonal activity of this species. Considering the daily activity, we observed a tendency of snakes to avoid the warmest hours. Bothrops jararacussu tend to avoid open areas, being registered only inside and at the edges of the forest. We compared our results with previous studies realized at tropical areas and we suggest the observed seasonal activity as an evolutive response, despite the influence of the different environmental variables, according to the occurence region.

Efficiency of snake sampling methods in the Brazilian semiarid region.

The choice of sampling methods is a crucial step in every field survey in herpetology. In countries where time and financial support are limited, the choice of the methods is critical. The methods used to sample snakes often lack objective criteria, and the traditional methods have apparently been more important when making the choice. Consequently researches using not-standardized methods are frequently found in the literature. We have compared four commonly used methods for sampling snake assemblages in a semiarid area in Brazil. We compared the efficacy of each method based on the cost-benefit regarding the number of individuals and species captured, time, and financial investment. We found that pitfall traps were the less effective method in all aspects that were evaluated and it was not complementary to the other methods in terms of abundance of species and assemblage structure. We conclude that methods can only be considered complementary if they are standardized to the objectives of the study. The use of pitfall traps in short-term surveys of the snake fauna in areas with shrubby vegetation and stony soil is not recommended.

The role of phytophysiognomies and seasonality on the structure of ground-dwelling anuran (Amphibia) in the Pampa biome, Southern Brazil.

Considering that habitat use by amphibians is related both with climate and environmental features, we tested the hypothesis that anuran assemblages found in different phytophysiognomies and in different seasons vary in structure. Additionally, we searched for species which can be indicators of habitat and seasons. The study was conducted in the Pampa biome, southern Brazil. Sampling was done through pitfall traps placed in three phytophysiognomies: grassland, ecotone grassland/forest; and forest. The seasonality factor was created by grouping months in warn and cold seasons. Sixteen species were found and the assemblages were influenced both by phytophysiognomies and climatic seasonality. In a paired comparison, the three phytophysiognomies differed in structure of assemblage from each other. Physalaemus henselii, P. riograndensis, Pseudopaludicola falcipes and Pseudis minuta were indicators of ecotone. Leptodactylus gracilis and Physalaemus biligonigerus were indicators of grassland. None species was indicator of forest. Most of the species were indicators of warm season: Elachistocleis bicolor, Leptodactylus fuscus, L. gracilis, L. latinasus, L. latrans, L. mystacinus, Physalaemus biligonigerus, P. cuvieri and Pseudis minuta. None species was indicator of cold season. We found that even for species of open areas, as Pampa, heterogeneous phytophysiognomies are important for maintaining abundance and constancy of populations of anuran.

Ecologia e história natural das serpentes de uma área de Caatinga no nordeste brasileiro / Ecology and natural history of snakes in a area of Caatinga in northeastern Brazil

We studied a snake assemblage from an area of Caatinga with shrub and tree vegetation to describe the natural history of the snake species. A total of 636 individuals among 22 species from four families were recorded. The distribution of species abundances is log-normal; and the composition presents typical species from the Caatinga, with Oxybelis aeneus (Wagler, 1824) and Philodryas nattereri Steindachner, 1870 being the most common. The natural history of each species is described based on information regarding activity patterns, diet, habitat use, reproduction, and defensive repertoire obtained during the study, as well as on information available in the literature. The study area is located in a priority region for conservation and our results emphasize that conservation policies should be implemented in the region.(au)

Pampean lizard assemblage from subtropical Brazil: a temporal analysis.

The increasing human occupation of natural environments is one of the greatest threats to biodiversity. To mitigate the negative anthropogenic effects, it is necessary to understand the characteristics of natural populations and the natural history of species. A study was conducted with an assemblage of lizards from a disturbed area of the Pampa biome, from February 2001 to January 2004. The assemblage showed a unimodal seasonal pattern, with the recruitment period occurring during the warmer months. The captures were seasonal for two of the three monitored years, and concentrated within warmer months. The minimum temperature explained the number of catches for the assemblage as a whole. However, when the species were analyzed individually, the temperature only explained the seasonal occurrence of Teius oculatus. The abundance of species was significantly different in the third year of study for Cercosaura schreibersii and Ophiodes striatus. This latter species was no longer registered in the study area from May 2003 until the end of the study. Therefore, O. striatus may be more sensitive to environmental changes, considering the events of change in vegetation during the study. With frequent and increasing environmental disturbances, it is necessary to take conservation measures and encourage the increase of knowledge on Pampean lizards.

Pampean lizard assemblage from subtropical Brazil: a temporal analysis

The increasing human occupation of natural environments is one of the greatest threats to biodiversity. To mitigate the negative anthropogenic effects, it is necessary to understand the characteristics of natural populations and the natural history of species. A study was conducted with an assemblage of lizards from a disturbed area of the Pampa biome, from February 2001 to January 2004. The assemblage showed a unimodal seasonal pattern, with the recruitment period occurring during the warmer months. The captures were seasonal for two of the three monitored years, and concentrated within warmer months. The minimum temperature explained the number of catches for the assemblage as a whole. However, when the species were analyzed individually, the temperature only explained the seasonal occurrence of Teius oculatus. The abundance of species was significantly different in the third year of study for Cercosaura schreibersii and Ophiodes striatus. This latter species was no longer registered in the study area from May 2003 until the end of the study. Therefore, O. striatus may be more sensitive to environmental changes, considering the events of change in vegetation during the study. With frequent and increasing environmental disturbances, it is necessary to take conservation measures and encourage the increase of knowledge on Pampean lizards.

O crescimento da ocupação humana sobre ambientes naturais é uma das maiores ameaças à biodiversidade. Para amenizar os efeitos negativos antropogênicos, é necessário entender as características das populações naturais, e a história natural das espécies. Um estudo foi conduzido com uma assembeia de lagartos de uma área perturbada do Pampa, de fevereiro de 2002 a janeiro de 2004. A assembleia apresentou padrão sazonal unimodal, com recrutamento ocorrendo durante os meses mais quentes. As capturas foram sazonais durante dois dos três anos monitorados, e concentradas nos meses mais quentes. A temperatura mínima explica o número de capturas para a assembleia como um todo. Entretanto, quando as espécies foram analisadas individualmente, a variável explica apenas a ocorrência sazonal de Teius oculatus. A abundância das espécies foi significativamente distinta no terceiro ano de estudo para Cercosaura schreibersii e Ophiodes striatus. Essa última espécie não teve mais registros na área de maio de 2003 até o final do estudo. Assim, O. striatus pode ser mais sensível às udanças abientais, considerando os eventos de udança na vegetação ocorridas durante o estudo. Com as frequentes e crescentes perturbações ambientais que o bioma vem sofrendo, são necessárias ações de conservação, e dar suporte para o aumento do conhecimento dos lagartos pampeanos.